小鼠胰岛素（Insulin）酶联免疫分析（ELISA）试剂盒使用说明书
本试剂仅供研究使用目的：本试剂盒用于测定小鼠血清样本中胰岛
素（Insulin）含量。
实验原理：
本试剂盒应用双抗体夹心法测定标本中小鼠胰岛素（Insulin）水平。用纯化的小鼠胰岛素（Insulin）抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入胰岛素（Insulin），再与HRP 标记的胰岛素（Insulin）抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物TMB 显色。TMB 在HRP 酶的催化下转化成蓝色，并在酸的作用下转化成*终的黄色。颜色的深浅和样品中的胰岛素（Insulin）呈正相关。用酶标仪在450nm 波长下测定吸光度（OD 值），通过标准曲线计算样品中小鼠胰岛素（Insulin）含量。
试剂盒组成：
试剂盒组成48 孔配置96 孔配置保存
说明书1 份1 份
封板膜2 片（48） 2 片（96）
密封袋1 个1 个
酶标包被板1×48 1×96 2-8℃保存
标准品：18 mIU/L 0.5ml×1 瓶0.5ml×1 瓶2-8℃保存
标准品稀释液1.5ml×1 瓶1.5ml×1 瓶2-8℃保存
酶标试剂3 ml×1 瓶6 ml×1 瓶2-8℃保存
样品稀释液3 ml×1 瓶6 ml×1 瓶2-8℃保存
显色剂A 液3 ml×1 瓶6 ml×1 瓶2-8℃保存
显色剂B 液3 ml×1 瓶6 ml×1 瓶2-8℃保存
终止液3ml×1 瓶6ml×1 瓶2-8℃保存
浓缩洗涤液（20ml×20 倍）×1 瓶（20ml×30 倍）×1 瓶2-8℃保存
样本处理及要求：
1. 血清：室温血液自然凝固10-20 分钟，离心20 分钟左右（2000-3000 转/分）。仔细收集上清，保存过程中如出现沉淀，应再次离心。
2. 血浆：应根据标本的要求选择EDTA、柠檬酸钠或肝素作为抗凝剂，混合10-20 分钟后，离心20 分钟左右（2000-3000 转/分）。仔细收集上清，保存过程中如有沉淀形成，应该再次离心。
3. 尿液：用无菌管收集，离心20 分钟左右（2000-3000 转/分）。仔细收集上清，保存过程
中如有沉淀形成，应再次离心。胸腹水、脑脊液参照实行。
4. 细胞培养上清：检测分泌性的成份时，用无菌管收集。离心20 分钟左右（2000-3000 转/分）。仔细收集上清。检测细胞内的成份时，用PBS（PH7.2-7.4）稀释细胞悬液，细胞浓度达到100 万/ml 左右。通过反复冻融，以使细胞破坏并放出细胞内成份。离心20 分钟左右（2000-3000 转/分）。仔细收集上清。保存过程中如有沉淀形成，应再次离心。
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5. 组织标本：切割标本后，称取重量。加入一定量的PBS，PH7.4。用液氮迅速冷冻保存备用。标本融化后仍然保持2-8℃的温度。加入一定量的PBS（PH7.4），用手工或匀浆器将标本匀浆充分。离心20 分钟左右（2000-3000 转/分）。仔细收集上清。分装后一份待检测，其余冷冻备用。
6. 标本采集后尽早进行提取，提取按相关文献进行，提取后应尽快进行实验。若不能马上进行试验，可将标本放于-20℃保存，但应避免反复冻融.
7. 不能检测含NaN3 的样品，因NaN3 抑制辣根过氧化物酶的（HRP）活性。
操作步骤
1. 标准品的稀释与加样：在酶标包被板上设标准品孔10 孔，在、第二孔中分别加标准品100μl，然后在、第二孔中加标准品稀释液50μl，混匀；然后从孔、第二孔中各取100μl 分别加到第三孔和第四孔，再在第三、第四孔分别加标准品稀释液50μl，混匀；然后在第三孔和第四孔中先各取50μl 弃掉，再各取50μl 分别加到第五、第六孔中，再在第五、第六孔中分别加标准品稀释液50ul，混匀；混匀后从第五、第六孔中各取50μl 分别加到第七、第八孔中，再在第七、第八孔中分别加标准品稀释液50μl，混匀后从第七、第八孔中分别取50μl 加到第九、第十孔中，再在第九第十孔分别加标准品稀释液50μl，混匀后从第九第十孔中各取50μl 弃掉。（稀释后各孔加样量都为50μl，浓度分别为12 mIU/L，8.0 mIU/L ，4.0 mIU/L，2.0 mIU/L，1.0 mIU/L）。
2. 加样：分别设空白孔（空白对照孔不加样品及酶标试剂，其余各步操作相同）、待测样品孔。在酶标包被板上待测样品孔中先加样品稀释液40μl，然后再加待测样品10μl（样品*终稀释度为5 倍）。加样将样品加于酶标板孔底部，尽量不触及孔壁，轻轻晃动混匀。
3. 温育：用封板膜封板后置37℃温育30 分钟。
4. 配液：将30（48T 的20 倍）倍浓缩洗涤液用蒸馏水30（48T 的20 倍）倍稀释后备用。
5. 洗涤：小心揭掉封板膜，弃去液体，甩干，每孔加满洗涤液，静置30 秒后弃去，如此重复5 次，拍干。
6. 加酶：每孔加入酶标试剂50μl，空白孔除外。
7. 温育：操作同3。
8. 洗涤：操作同5。
9. 显色：每孔先加入显色剂A50μl，再加入显色剂B50μl，轻轻震荡混匀，37℃避光显色15 分钟.
10. 终止：每孔加终止液50μl，终止反应（此时蓝色立转黄色）。
11. 测定：以空白空调零，450nm 波长依序测量各孔的吸光度（OD 值）。测定应在加终止液后15 分钟以内进行。
注意事项：
1． 试剂盒从冷藏环境中取出应在室温平衡15-30 分钟后方可使用，酶标包被板开封后如未用完，板条应装入密封袋中保存。
2． 浓洗涤液可能会有结晶析出，稀释时可在水浴中加温助溶，洗涤时不影响结果。
3． 各步加样均应使用加样器，并经常校对其准确性，以避免试验误差。一次加样时间控制在5 分钟内，如标本数量多，推荐使用排枪加样。
4． 请每次测定的同时做标准曲线，做复孔。如标本中待测物质含量过高（样本OD 值大于标准品孔孔的OD 值），请先用样品稀释液稀释一定倍数（n 倍）后再测定，计算时请*后乘以总稀释倍数（×n×5）。
5． 封板膜只限一次性使用，以避免交叉污染。
6． 底物请避光保存。
7． 严格按照说明书的操作进行，试验结果判定必须以酶标仪读数为准.
8． 所有样品，洗涤液和各种废弃物都应按传染物处理。
9． 本试剂不同批号组分不得混用。
10. 如与英文说明书有异，以英文说明书为准。
计算：
以标准物的浓度为横坐标，OD 值为纵坐标，
在坐标纸上绘出标准曲线，根据样品的OD
值由标准曲线查出相应的浓度；再乘以稀释
倍数；或用标准物的浓度与OD 值计算出标
准曲线的直线回归方程式，将样品的OD 值
代入方程式，计算出样品浓度，再乘以稀释
倍数，即为样品的实际浓度。
（此图仅供参考）
试剂盒性能：
1.样品线性回归与预期浓度相关系数R 值为0.95 以上。
2.批内与批见应分别小于9%和11%
检测范围：
8.0 mIU/L -15 mIU/L
保存条件及有效期：
1.试剂盒保存：；2-8℃。
2．有效期：6 个月
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Mouse Insulin
FOR RESEARCH USE ONLY
Drug Names
Generic Name：Mouse Insulin ELISA Kit.
Purpose
This kit allows for the determination of Insulin concentrations in Mouse serum ａnd other
biological fluids.
Principle of the assay
The kit assay Mouse Insulin level in the sample, use Purified Mouse Insulin antibody to
coat microtiter plate wells, make solid-phase antibody, then add Insulin to wells, Combined
Insulin antibody which With HRP labeled, become antibody - antigen - enzyme-antibody
complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue
color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid
solution ａnd the color change is measured spectrophotometrically at a wavelength of 450 nm.
The concentration of Mouse Insulin in the samples is then determined by comparing the O.D. of
the samples to the standard curve.
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Materials provided with the kit
Materials provided with
the kit
48determinations 96 determinations Storage
User manual 1 1
Closure plate membrane 2 2
Sealed bags 1 1
Microelisa stripplate 1 1 2-8℃
Standard：18 mIU/L 0.5ml×1 bottle 0.5ml×1 bottle 2-8℃
Standard diluent 1.5ml×1 bottle 1.5ml×1 bottle 2-8℃
HRP-Conjugate reagent 3ml×1 bottle 6ml×1 bottle 2-8℃
Sample diluent 3ml×1 bottle 6ml×1 bottle 2-8℃
Chromogen Solution A 3ml×1 bottle 6ml×1 bottle 2-8℃
Chromogen Solution B 3ml×1 bottle 6ml×1 bottle 2-8℃
Stop Solution 3ml×1 bottle 6ml×1 bottle 2-8℃
wash solution
（20ml×20 fold）
×1bottle
（20ml×30 fold）
×1bottle
2-8℃
Specimen requirements
1. serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of
2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
2. plasma-use suited EDTA, citrate or heparinized plasma as an anticoagulant,mix 10-20
mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If
precipitation appeared, Centrifugal again.
3. Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m.
remove supernatant, If precipitation appeared, Centrifugal again. The Operation of
Hydrothorax ａnd cerebrospinal fluid Reference to it.
4. cell culture supernatant-detect secretory components, collect sue a sterile container,
centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the
composition of cells, Dilut cell suspension with PBS （ PH7.2-7.4 ） , Cell concentration
reached 1 million / ml, repeated freeze-thaw cycles, damage cells ａnd release of
intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove
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supernatant, If precipitation appeared, Centrifugal again.
5. Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly
frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）,
Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m.
remove supernatant.
6. extract as soon as possible after Specimen collection,and according to the relevant
literature, ａnd should be experiment as soon as possible after the extraction. If it can’t,
specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.
7. Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.
Assay procedure
1.Dilute ａnd add sample to Standard: set 10 Standard wells on the ELISA plates coated, add
Standard 100μl to the first ａnd the second well, then add Standard dilution 50μl to the first and
the second well, mix; take out 100μl form the first ａnd the second well then add it to the third
and the forth well separately. then add Standard dilution 50μl to the third ａnd the forth
well ,mix ; then take out 50μl from the third ａnd the forth well discard, add 50μl to the fifth and
the sixth well ,then add Standard dilution 50μl to the fifth ａnd the sixth well, mix ; take out 50μl
from the fifth ａnd the sixth well ａnd add to the seventh ａnd the eighth well, then add Standard
dilution 50μl to the seventh ａnd the eighth well ,mix ; take out 50μl from the seventh ａnd the
eighth well ａnd add to the ninth ａnd the tenth well, add Standard dilution 50μl to the ninth and
the tenth well, mix , take out 50μl from the ninth ａnd the tenth well discard（add Sample 50μl to
each well after Diluting ,（density: 12 mIU/L,8.0 mIU/L ,4.0 mIU/L,2.0 mIU/L,1.0 mIU/L）
2.add sample ： Set blank wells separately （blank comparison wells don’t add sample and
HRP-Conjugate reagent, other each step operation is same）. testing sample well. add Sample
dilution 40μl to testing sample well, then add testing sample 10μl （sample final dilution is
5-fold）, add sample to wells , don’t touch the well wall as far as possible, ａnd Gently mix.
3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.
4.Configurate liquid: 30-fold （or 20-fold）wash solution diluted 30-fold （or 20-fold） with distilled
water ａnd reserve.
5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer
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to every well, still for 30s then drain, repeat 5 times, dry by pat.
6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except blank well.
7.incubate：Operation with 3.
8.washing：Operation with 5.
9.color：Add Chromogen Solution A 50ul ａnd Chromogen Solution B to each well, evade the
light preservation for 15 min at 37℃
10.Stop the reaction ： Add Stop Solution50μl to each well, Stop the reaction（the blue color
change to yellow color）.
11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution and
within 15min.
Important notes
1. The kit takes out from the refrigeration environment should be balanced 15-30 minutes in
the room temperature, ELISA plates coated if has not use up after opened, the plate should
be stored in Sealed bag.
2. washing buffer will Crystallization separation, it can be heated the water helps dissolve
when dilute . Washing does not affect the result.
3. add Sample with sampler Each step, And proofread its accuracy frequently, avoids the
experimental error. add sample within 5 min, if the number of sample is much , recommend
to use Volley .
4. if the testing material content is excessively higher （The sample OD is bigger than the first
standard well ）,please dilute Sample （n-fold）, Please diluente ａnd multiplied by the dilution
factor.（×n×5）.
5. Closure plate membrane only limits the disposable use, to avoid cross-contamination.
6. The substrate evade the light preservation.
7. Please according to use instruction strictly, The test result determination must take the
microtiter plate reader as a standard.
8. All samples, washing buffer ａnd each kind of reject should according to infective material
process.

原创作者：上海远慕生物科技有限公司