产品简介: Backbone pComb3X containing PCR template for human antibody lambda light chain constant region. pComb3X is the newest of the pComb vectors. Improvements over pComb3 include increased stability ａnd introduction of an asymmetric SfiI cassette for directional cloning of full Fab, scFv, peptide ａnd other protein for phage display. 6xHis ａnd HA tags allow for purification ａnd detection. An amber stop codon was introduced to turn-off expression of the pIII fusion protein by switching to a non-supressor strain of E. coli allowing production of soluble protein without subcloning. Alternatively, the gene for phage protein pIII can be removed by SpeI/NheI digest. pComb3XLambda can be used as a template to amplify the lambda light chain constant region for construction of chimeric antibody libraries as described in: Barbas, C. F., III; Burton, D. R.; Scott, J.K., Silverman, G.J. Eds. (2001) Phage Display: A Laboratory Manual; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, New York Please note that there is a A246V mutation in heavy chain region; however, this vector is meant to be used as a PCR template for the light chain region from base# 613-1016.
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