pET-32C质粒是一个大肠杆菌表达载体，T7启动子驱动TrxA促溶标签和目的蛋白融合表达。 The pET-32 series is designed for cloning ａnd high-level expression of peptide sequences fused with the 109aa Trx•Tag thioredoxin protein . Cloning sites are available for producing fusion proteins also containing cleavable His•Tag ａnd S•Tag sequences for detection ａnd purification. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circle map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, single-stranded sequencing should be performed using the T7 terminator primer .
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